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KMID : 1007520120210030911
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Food Science and Biotechnology
2012 Volume.21 No. 3 p.911 ~ p.916
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Differentiation of Lactic Acid Bacteria Based on RFLP Analysis of the tuf Gene
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Park Seok-Hwee
Jung Jong-Hyun
Seo Dong-Ho
Lee Hye-Lim
Kim Gye-Won
Park Sun-Young
Shin Woo-Chang
Hong Sung-Youl
Park Cheon-Seok
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Abstract
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The tuf gene, which encodes the elongation factor Tu, is universally distributed in Gram-positive bacteria. A small region of the tuf gene was evaluated for the differentiation of Lactobacillus strains using restriction fragment length polymorphism (RFLP) analysis. The 803bp portion of tuf genes from 9 closely-related Lactobacillus reference strains were successfully amplified by polymerase chain reaction (PCR), and restriction patterns of each were evaluated with a set of restriction enzymes, which included AluI, HaeIII, and Sau3AI. The results implied that restriction patterns obtained from 2 of these restriction enzymes (AluI and HaeIII) could effectively differentiate closely-related Lactobacillus species.
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KEYWORD
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Lactobacillus, tuf gene, restriction fragment length polymorphism (RFLP), lactic acid bacteria
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